dominant negative tcf4 dntcf4 constructs (Addgene inc)
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Addgene inc
dominant negative tcf4 dntcf4 constructs
Dominant Negative Tcf4 Dntcf4 Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dominant negative tcf4 dntcf4 constructs/product/Addgene inc
Average 91 stars, based on 5 article reviews
Dominant Negative Tcf4 Dntcf4 Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dominant negative tcf4 dntcf4 constructs/product/Addgene inc
Average 91 stars, based on 5 article reviews
dominant negative tcf4 dntcf4 constructs - by Bioz Stars,
2026-06
91/100 stars
Images
1) Product Images from "Targeting Wnt/β-catenin signaling enhances the efficacy of anti-CD38 immunotherapy in multiple myeloma"
Article Title: Targeting Wnt/β-catenin signaling enhances the efficacy of anti-CD38 immunotherapy in multiple myeloma
Journal: Neoplasia (New York, N.Y.)
doi: 10.1016/j.neo.2025.101242
Figure Legend Snippet: Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either dnTCF4 or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.
Techniques Used: Inhibition, Western Blot, Expressing, Transduction, Plasmid Preparation, Control, CRISPR
Figure Legend Snippet: Inhibition of Wnt signaling upregulates CD38 expression in MM. (A) Analysis of CD38 mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (48 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. * p ≤ 0.05; *** p ≤ 0.001 using Student’s t-test. (B) Flow cytometry analysis of cell-surface expression of CD38 in control empty vector-transduced (EV control) and dnTCF4-transduced HMCLs (48 h after transduction). A representative plot of three independent experiments is shown. (C) Flow cytometry analysis of cell-surface expression of CD38 in control empty vector-transduced (EV control) and dnTCF4-transduced HMCLs (48 h after transduction), and mean fluorescence intensity (MFI) is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Analysis of CD38 mRNA expression in the HMCLs after ICG-001 (5 µM) treatment for 24 h by qPCR. The mean ± SD of three independent experiments in triplicate is shown. * p ≤ 0.05; ** p ≤ 0.01 using Student’s t-test. (E) Flow cytometry analysis of HMCLs cell-surface CD38 expression after ICG-001 (5 µM) treatment for 24 h.
Techniques Used: Inhibition, Expressing, Transduction, Plasmid Preparation, Flow Cytometry, Control, Fluorescence